In December of 1947 Carl Barks introduces Uncle Scrooge in Christmas on Bear Mountain in Four Color #178.

Gomer: It's damp under there. Could be spiders.

Barney: Now what makes you think there's spiders under there?

Gomer: Cause... If I were a spider that's where I'd go.

Barney: Gomer there are no spiders under there. now get movin'.

Gomer: I don't know. Sure looks pretty spidery to me.

Barney: ...Gomer, get down there with them spiders and start workin'.